Methylene Blue CP 500g
Methylene Blue CP in a lab is primarily used as a staining agent for microscopy to enhance the visibility of cells and tissues, and as a redox indicator in analytical chemistry.
Here’s a more detailed breakdown:
1. Staining Agent for Microscopy:
Cell Viability:
Methylene blue can be used to determine cell viability, as healthy cells reduce the stain, causing it to lose its color.
Tissue staining:
It stains various tissues and cells, helping to visualize their morphology and structure under a microscope.
Specific Applications:
Staining blood films/smears used in cytology.
Staining RNA or DNA for viewing under the microscope or on hybridization membranes.
Staining human amniotic fluid stem cells to determine cell viability.
Staining bacteria and leukocytes.
2. Redox Indicator in Analytical Chemistry:
Detection of Oxidizing Agents: Methylene blue solutions are blue in oxidizing environments but turn colorless when exposed to reducing agents.
Analytical Chemistry Applications: It’s used to detect the presence or absence of oxygen and other oxidizing agents.
Surfactant Detection: Methylene blue can be used to detect anionic surfactants in water samples.
3. Other Laboratory Uses:
Water Testing: Methylene blue can be used to detect surfactants in water samples.
Photosensitizer: Methylene blue can be used as a photosensitizer for the trace analysis of anionic surfactants
Synonyms : Â methylthioninium chloride ,CI 52015, basic blue 9
Application :Â Â it is mainly used to treat methemoglobinemia. Specifically, it is used to treat methemoglobin levels that are greater than 30% or in which there are symptoms despite oxygen therapy.
Methylene Blue CP 500g
