Lactose Broth 500g
Lactose broth is primarily used in microbiology labs to detect and identify coliform bacteria, including E. coli, in water, food, and dairy products. It’s also used as a pre-enrichment medium to increase the number of target bacteria, like Salmonella, before more specific testing.
Here’s a more detailed look at Lactose Broth 500g uses:
1. Detection of Coliform Bacteria:
Lactose broth is a rich medium containing lactose, a sugar that coliform bacteria can ferment, producing acid and gas.
The fermentation of lactose, often with gas production, is a key characteristic used to identify coliforms.
The broth is used in a “presumptive test” for coliforms, where a positive result (gas production or acid production) suggests the presence of coliforms.
The American Public Health Association (APHA) recommends lactose broth for the presumptive test of coliform bacteria in water, food, and milk.
2. Pre-Enrichment for Salmonella:
Lactose broth can be used to increase the number of Salmonella bacteria in a sample before further testing, making them easier to detect.
While Salmonella does not typically ferment lactose, the broth provides a non-selective environment for its growth.
This pre-enrichment step is particularly useful when Salmonella is present in low numbers.
3. Other Uses:
Lactose broth can be used in conjunction with other media for further identification of bacterial species.
It can also be used in the analysis of food, milk products, and water for Salmonella.
Lactose Broth is used for the detection of coliform bacteria in water, foods and dairy products.
Lactose Broth is recommended by the American Public Health Association (APHA), for the presumptive test of coliform bacteria in water, food and milk.
Lactose Broth 500g
